The rice and wheat protocols are quite effective in rice and wheat protoplast transformation, and transformation efficiency reaches 58.4 and 64.5%, respectively, confirmed by flow cytometry detection. ![]() To establish a PEG-mediated banana protoplast transformation system, we optimized the transformation method based on transformation protocols for rice and wheat. This system was used to successfully deliver RNPs into banana protoplasts, and the RNP system was detected to be working through deep amplicon sequencing, laying a good foundation for the further study of banana genome editing.Įstablishment of a PEG-mediated protoplast transformation system in banana In this study, a PEG-mediated transformation system was established, which provided an effective method for the detection of gRNA activity. To date, several investigations have reported the application of CRISPR-Cas9 gene editing technology in bananas, yet transgenic plants containing T-DNA were generated in each of these cases of CRISPR/Cas9 gene editing, and a DNA-free genome editing method has not been developed in banana. successfully transferred RNP into protoplasts from grapes and apples, and the mutation efficiency was as high as 6.9%. The genome-edited mutant regenerated from protoplasts contained no transgenic ingredients. directly transferred RNP into the protoplasts of Arabidopsis thaliana, tobacco and rice for the first time. To this date, mature and stable genetic transformation systems for protoplast transient expression have been established in Arabidopsis, wheat, rice, maize and other species.Ĭurrently, there are many reports on PEG-mediated protoplast transformation of DNA, but few reports on PEG-mediated protoplast transformation of ribonucleoprotein (RNP). Among these, the PEG-mediated method is widely used due to its easy operation, low cost, lack of requirements for specific equipment and generation of stable results. The common methods used for plant protoplast transformation include PEG-mediated transformation, electroporation-mediated transformation and microinjection-based transformation. The transient transformation system of plant protoplasts without unique cell wall characteristics is extensively used in genetic research involving gene function identification, subcellular localization and gene editing. This method was widely used because of its high yield, high activity, easy operation and wide adaptability. ![]() In 1960, Cocking successfully isolated tomato root tip protoplasts for the first time by enzymatic hydrolysis. As early as 1892, Klercker obtained protoplasts by a mechanical method, which resulted in a low yield and suffered from difficult operation and poor applicability. The application of the CRISPR/Cas9 RNP system enables the generation of banana plants engineered by DNA-free gene editing.Ī protoplast is a cell of a plant, fungus, bacterium, or archaeon from which the cell wall has been removed by plasmolysis, leaving the protoplasm and plasma membrane. The PEG-mediated banana protoplast transformation system can serve as a rapid and effective tool for transient expression assays and sgRNA validation in banana. Deep amplicon sequencing results showed that the editing efficiency of the CRISPR/Cas9 system was higher than that of the other two systems. Specific bands were observed in PCR-Restriction Enzyme Digestion (PCR-RE) assays, and Sanger sequencing of single clones further confirmed the occurrence of indels at target sites. This system was further optimized for successfully delivering CRISPR/Cas9 and CRISPR/Cas12a plasmids and CRISPR/Cas9 ribonucleoproteins (RNPs) for targeted delivery of the PDS gene into banana protoplasts. ![]() In this study, we established a PEG-mediated banana protoplast transformation system. Protoplasts constitute a versatile platform for transient expression in plant science. Here, the establishment of a PEG-mediated banana protoplast transformation system makes it possible to build an efficient DNA-free method for a site-directed mutagenesis system. To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana.
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![]() ![]() This will make it a lot easier for the search party to find the lost individual thus speeding up the process of the rescue operation. the latitude and longitude of the signal's origin location using the GPS. So with that not only Savior can send the SOS signal using radio, but Saviour sends the location details i.e. Since GPS satellites revolve all around the world, they can connect and get your current location. Now, it also contains a GPS that can get your direct coordinates. It also has a buzzer to sound, how the Code will sound. It also has an OLED display, that can translate any sentence to its Morse Code, and display that code on the OLED simultaneously transmitting the code over the air, and also flashing it via a LED. Saviour uses Morse Code signaling via a radio transmitter, also with a LED. If one gets stuck in a deserted area, say on an island or some mountain, where the Mobile gets useless, due to no network coverage, radios are still there, which can be a very effective way of communication in that scenario, and still, they are used all way. Saviour can do radio signalings like the popular SOS signal or any emergency signaling. The idea behind Saviour was to create a hack that allows people to travel to remote locations without having to worry about getting lost or getting stuck there.What it doesSaviour is an emergency tool that is very powerful, in times of real juncture. where the protagonist gets stuck on an island, even though it sounds very adventurous, but nonetheless the dangerous probability of it happening in real to people still remains. SaviourInspirationThe countless stories and movies like the Lord of the Flies, Robinson Crusoe, Titanic, Cast Away, Six Days Seven Nights, etc. ![]() I am not the original author of this circuit.Thanks to: A short SW1 press exist to the display current time mode.While setting the current time or alarm in 12-hour mode, the colon will be off to indicate AM and is on to indicate PM.Circuit diagram and bill of materials available in the download section.P.S. A short SW2 press will toggle this setting. Setting the alarm is similar to setting the current time.A short SW1 press while viewing the alarm or exiting out of setting the alarm will enter the enable/disable alarm mode. While viewing the alarm time, a long press of SW1 will enter the set alarm mode, at which point only the digits being set will blink. This is done to differentiate between viewing the current time and the currently set alarm time. When viewing the currently set alarm time, the display will blink. Another SW1 press will exit set time mode and the clock will return to display the current time.Short SW1 presses cycle through setting and enabling the alarm. A short SW1 press will then move to the minutes where operation is similar to setting the hour. Holding SW2 down will cause the clock to increment quickly after 1 second. ![]() Short SW2 presses will increment the hour by 1. Timer currently resets after 60 minutes.A long SW1 press while viewing the current time in HH:MM format will enter the set time mode. The timer will continue to increment (if running) even when cycling through other modes. A short SW1 press will toggle the start/stop of the timer. A short SW2 press will then enter the timer mode. A short SW1 press while on the 12/24-hour display format will toggle the setting. More information (part lists, (YouTube) tutorials, configuration examples, etc.) can be found on !Ĭircuit OperationShort SW2 presses cycle through displaying the current time in HH:MM and MM:SS format as well as the 12/24-hour display format option. Fully 12v and 24v compatible with a max input voltage of 36v for running other loads (flyback diodes and capacitor optional for inductive loads!).Using a pluggable ESP32 and the QuinLED-ESP32 the board can be configured with an ESP32 with onboard antenna, external antenna or even wired Ethernet!! Please note that to be able to build this board you will require to order a stencil with it !Total cost per board with components is less than xx.xx$. The board has specifically been designed for high power setups where you might want to run several high powered white LED strips too, allowing a max of 10Amps over a single MOSFET or ~20Amps for the total board. The QuinLED-An-Penta is a 5 channel ESP32 based analog PWM LED controller/dimmer! Designed to run WLED or ESPhome it is the ideal board for running LEDs standalone using an App or tied into Home Assistant natively! Designed to build yourself, easy to solder!Unique properties are high current handling, 2x onboard fuse, 4x Positive and 5x Negative (LED channels) terminals to run any combination of LED setup from RGB, RGBW to RGB+CCT (RGB + Dual White) all possible. ![]()
Many of the games require no downloading or installing and can be played in the browser just like Flash but using Java while others require a free and safe plugin to be installed. and also have 100s of hacked roms, all playable on the site hassle free. RGR offers games of every genre including RPG, Platform, Arcade, Sports, Puzzle, Strategy, Simulation, Board, Card, Multiplayer, etc. Play Games from Retro Classic gaming systems including Super Nintendo, Sega Genesis, Game Boy Advance, Atari, etc and from the greatest franchises including Mario, Pokemon, Sonic, Zelda, Final Fantasy, Mega Man, etc. It’s definitely worth the watch.Vizzed Retro Game Room offers 1000s of free professionally made games, all playable online on the website. I wasn’t disappointed for the most part and it made me think a lot harder about all the times I’ve been to the rough side of town in my own community. Snow On Tha Bluff has a quick conclusion that left a bad taste in my mouth, but as my boyfriend Dustin said “I mean isn’t it supposed to be that way? Yeah, some serious shit just went down but life for him just moves right along.”Īll in all, I did really like this movie. One just has to sit down to this movie with an open mind and hope that some of the more unpleasant scenes were staged. The controversy sparked by this movie isn’t shocking, everything about the subject matter is controversial. Social injustice is not what this film is about, it’s just about Curtis Snow. Many statements about social issues could be considered, but they’re more or less pointed out in passing. This just leads to the notion about how quickly life moves for Snow and the ones around him. You could say the movie is hard to follow, if you zone out for a few seconds you’ll come back confused as to what’s going on. He’s unabashedly selfish and narcissistic, but that’s exactly who he is and that’s nothing he’ll ever apologize for. His way of showing love for his mother was to sell crack to her instead of letting her get it from the street. When his grandmother tries to talk him out of the dope game he pretends to listen and busts out laughing as soon as he steps out of her house. His loved ones dying in the street is just how things are, so is going to jail. He sees nothing wrong with slinging dope or armed robbery, it’s what provides for his son and baby mama. The shocking parts of this movie aren’t the times of extreme violence, but when Snow turns to the camera and speaks with complete honesty. (I did some research while watching the movie to verify all this since I have a habit of dismissing anything that’s supposed to be “found footage” as fake.) Turns out it’s about as real as you can get without anyone really getting shot or having a toddler exposed to crack cocaine (which is still questionable). ![]() They film in Snow’s home neighborhood, The Bluff, a destitute region of Atlanta with his real neighbors as the rest of the cast. He actually sought out filmmaker Damon Russell to tell his story in the most realistic way possible. Snow On Tha Bluff is a docudrama about real life drug dealer and career criminal Curtis Snow and his everyday life. ![]() I instantly erased everything I had just written and decided it had to be this week’s subject. I was going through Netflix the other night while working on the blogpost for the movie I originally set on doing when it came up as the first option in an oddly specific subcategory. I had heard of Snow On Tha Bluff from news about the Slamdance Film Festival sometime last year and dismissed it as a movie I’d never get to see unless it somehow fell into my lap even though it had an official release a few months ago. ![]() Install, from I.A. run kijen NativeInstruments_Keygen.exe, select a product, click on the Register button Since the second VR release of this version of the tool turned out to be unsuccessful again, you have to do something.Īfter installing the tool, the parameter values are incorrectly entered in 2 places, and even different ones. We fix it. ![]() ![]() Native Instruments Battery – professional drum sampler. The widespread use of this sampler is made possible by low system resource requirements, ease of use and compatibility with a huge number of sample formats. The highlight of Battery is the matrix, the dimension of which can be changed by the user depending on the number of loaded samples (up to 128 cells). Thanks to the color coding of cells, the possibility of multiple selection, customization of rows and columns of the matrix, it became even faster and more convenient for the user to work with the contents and parameters of the cells. The properties of the Battery cells have been supplemented with such parameters as articulation, humanization. The samples of each cell can be adjusted for length / speed (Time Expand / Compress).įactory library : Library BATTERY 4 focuses on electronics and hip-hop manufacturing, featuring 70 brand new contemporary sets. All 143 kits are loaded instantly. Find your drum sound, drag it into the slot, and start playing – the ultra-intuitive tagging system lets you quickly find the sounds you’re looking for. BATTERY 4 is compatible with your samples, whether you use factory sounds or create your own. It even remembers the history for each cell separately – you can immediately see the path that led you to each sound, even after working with other cells. ![]() Of course racing games aren't just working like this in today's videogame scene, but the main goal of this project was trying to recreate that A E S T H E T I C feeling that vaporwave everyday gives us. Since this is a test I decided to keep it simple by just putting a chronometer to record your lap times and try to do the lowest possible time. Palmy Sunrise - Another quite long race, placed in a seaside and palmy city at the dawn of a new day. Sunset at Countryside - A little longer race in a near-lake green countryside, while the sun is going down. Night City - The first made, a short race in the middle of a cool night lighted city. I put togheter some of my old assets and started working on this little racing game demo. Some days ago I saw the Bart on Resonance vine and video so I quickly decided to make something playable with the concept. Shit, I know somethin' happenin' 'cause everything's movin', baby Hey, Pop, come on, old muthafucka I'll dust yo' ass off, baby! Pip, pip pip, haha, shit *whistles* Hey, Pops, you got anything on Duke? Straighten me out, baby Yeah, nigga, I got somethin' for yo' ass, got some advice Better lay off that narcotic, nigga, that shit done made you null and void I ain't lyin', boy, what's wrong wit' you? Why don't you straighten up, get a job? Get a job! Muthafucka, you talkin' to The Kid, baby! Shit, I worked five years in a row when I was in the joint! Pressin' them muthafuckin' license plates! I'm a license plate pressin' muthafucka, too, baby! Shit, where a nigga gon' get a job out here pressing license plates?! Pop, nigga, listen to me! Don't you hit me no more, boy! I'll dust your ol' junkie ass off! You know I will, nigga! You alright to me, boy I'm just ashamed to see you like this! Ashamed to see me? What about this shit out here? Niggas is fuckin' with me, baby! Was I finished?I went to the Unemployment Bureau, baby Bitch, sittin' behind the desk, ugly muthafucka! Come tell me, talkin' 'bout, "You have a criminal record" I said, "I know that, bitch! I'm a criminal! Just tell where I'm gon' get a job, ugly bitch! I seen a better faces on iodine bottle!" Bitch made me mad, I vomited and shit on the floor I did, man, then make that nigga with that pistol down there clean it up! Nigga talkin' 'bout, "Clean up that vomit, muthafucka!" Hahahaha I said, "Fuck you, nigga, I ain't cleanin' that shit up!" He said, "You don't clean this shit up, I'll shoot yo' ass!" I said, "Well, who gon' clean up the blood, nigga?" Hahaha Went outside, man, I was cleaner than a broke dick dog, too, baby Saw a bitch was so fine, woo.About a year ago I discovered the magic world of Vaporwave music and was quite fascinated by its aesthetic: the pink colour, the Windows 98 look on clips, the non-sense low poly and coloured 3D figures, the image of a old busted car riding in the night city with some great piece of slowed 80's funk-jazz-anything music in the background. ![]() Hey, fool, you better slow that car down! Goddamn, don't come driving down through there like you crazy! This a neighborhood, this ain't no residential district! Wah-ha-hahaha, Jesus on my mind Yeah, nigga, I know Jesus! Shit, I remember when the boy got killed, that's for real, man It was on a Friday down at the railroad depot I tried to warn the nigga I said, "Boy, don't you go down there fuckin' with them Jews without no money!" Hey, ice cream boy, you wanna turn that goddamn noise off?! Don't nobody wanna hear that shit this time of morning! Well, jump on out then, nigga, you bad! I got somethin' for yo' ass! I'm a veteran, boy! I was in World War I! The battle of the Chateaubriand I got mustard gas wounds all over my body! Wa-ha Who's that boy? Goddamn it, look at that nigga Look at him, in the middle of the street, junkie muthafucka, look at him Nigga used to be a genius I ain't lyin', booked the numbers, didn't need paper or a pencil Now the nigga can't remember who he is "Say, nigga, get yo' ass out the street, boy!" *whistles* Move out the way, boy!Īh.what's happenin'?! What's happenin'. Winos don't get drunk like everybody else, though, right? Wino be directing traffic on Sunday morning That was my favorite thing about the wino 'Cause I used to-I didn't wanna go to church, so I'd hang wit' him Wino be standin'. ![]() It can also be beneficial to adjust the report body width and height to account for unwanted blank space. Either will allow you to change the page units, size, and margins for the report you wish to print out. You’ll need to adjust the properties of the page orientation, dimensions, and margins of your Word document using either the Property Page or Properties pane. How Can I Avoid Blank Pages When Printing Paginated Reports? Head into the printer Properties and look to see if a separator page option has been enabled. If you’ve exhausted all solutions provided and you still have a blank page after printing out your Word document, it’s possible that the Printer Properties are in need of tweaking. Blank Pages Still Appear Even After Deleting Them All Using The Solutions Above. ![]() Most blank pages are the result of extended paragraphs (clicking Enter too many times pushing the article forward without adding words), annoying page breaks, or the report body width exceeds the available page space. FAQs About Blank Pages in Word Document Why Do Blank Pages Occur in a Microsoft Word Document? In the Page Setup window, you can reduce the page margins forcing Word to remove the blank pages being added due to inserted tables.Ĭlick OK to finalize your changes. Navigate to the Layout tab, click Margins, and then select Custom Margins… at the bottom of the dropdown menu. Luckily, we can narrow things down a bit using custom margins. Resumes created in Microsoft Word can often be the culprits of this. This will cause the Enter key to always push onward to another page even if there is visible blank space on the page. Tables that have been inserted into a Microsoft Word document can often take up an entire page. Method 4: Delete A Blank Page In Microsoft Word Document By Reducing Margins This will remove all page breaks from your Microsoft Word document. Add the ^m to the “Find what:” box, remove it from the “Replace with:” box, and click the Replace All button. This will add a ^m to the “Replace with:” box. From the pop-up menu, click Manual Page Break. In the newly opened section of the window, click Special. ![]() While in the Find and Replace window, navigate to the Replace tab and click the More > button. Navigate to the Editing section and click Replace. In Word 2010 or higher, navigate to the Home tab. Removal of multiple page breaks will require a different approach than that of a single page break. With longer documents, it’s not uncommon to have multiple page breaks. Method 3: Remove Multiple Page Breaks In Microsoft Word Document In the View tab, locate the Document Views, or Views, section and click Draft. While in Microsoft Word, at the top of the window, navigate over to the View tab. To remove the created blank page, you’ll first need to view the line of the page break. While in Print Layout mode, a page break may have been inserted which would take up an entire page. Method 2: Delete A Blank Page In Microsoft Word Document via Single Page Break If you notice a paragraph symbol on the blank page, it indicates an empty paragraph likely brought on by clicking Enter too many times. You can press Ctrl + * to show/hide paragraph marks that indicate where a paragraph begins. It’s possible that this was the result of an empty paragraph.
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![]() Please click “Android” then the device model for example “Samsung Galaxy S7” under the “Profile” options,then select the profile optimized for Samsung devices.You are also allow to set the output “video size” and “video quality” in this windows,then click “OK” button to begin to created and convert the 2D video to 3D for your Samsung phone. Step 3.Setting Output Format of Video for Samsung Gear VR. Three:To return to the main window,you could click “Finish”.Īfter finish setting the preferred 3D format for video,simply click “Convert” button and go on,the program will pop up a new output setting windows. Two:To check the “Swap L/R Image” option, you could switch left and right eye’s image. One:You could drag the “3D Depth” control slider to set the depth. Tips of Setting the 3D Depth for the video: Step 2: The 2D to 3D Converter program support to different 3D video format for instance Anaglyph (Red/Cyan), Side by Side, Side by Side (Half-Width), Top and Bottom and Top and Bottom (Half-Height),you should click your selection under the preview area to choose an output mode. Then click Add to import your 2D or 3D videos. Step 1: Run the 2D to 3D Converter on your PC/Mac, click “2D to 3D” selection to open settings interface of program. Steps to convert videos to 3D SBS video for Gear VR For detailed information, please visit convert 2D to 3D video for VR. With the best 2D to 3D Converter,you can freely convert 2D videos to 3D for Gear VR with Galaxy S8/S8 Edge, Galaxy S7/S7 Edge, Galaxy S6/S6 Edge/S6 Edge+, Galaxy Note 7, Galaxy Note 6/5, etc of all popular formats,such as AVI,MPEG,WMV,MP4,AVCHD Video,MPEG2 HD Video,etc.In this case, 2D to 3D Converter help to solve convert 3D videos problem by converting Anaglyph (Red/Cyan), Side by Side, Side by Side (Half-Width),Top and Bottom or Top and Bottom (Half-Height) with outstanding output video quality.Supported Android devices and iOS devices including Samsung(Galaxy S8/S8 Edge, Galaxy S7/S7 Edge/S7 Edge+,Galaxy S6/S6 Edge/S6 Edge+,Galaxy Note 7/6/5),HTC EVO 3D, Sharp SH-12C Aquos 3D, LG Optimus 3D P920 iPhone 7,iPhone 6S(Plus),Pad Pro,iPad Air,iPad Mini,iPad 4/3/2,and other compatible 3D display mobile devices. Besides Samsung Gear VR, the program also enables you to convert 3D movies for viewing on other popular 3D virtual reality headsets such as HTC Vive, ANTVR, Oculus Rift, Google Cardboard, Zeiss VR One, etc. Convert 2D/3D Videos for Gear VR with Samsung Galaxy S8/S8 Edge, Galaxy S7/S6 (Edge) or Galaxy Note 7/6/5 On a Mac, files can be transferred to your phone using Android File Transfer ( ), which is a free utility provided by Google. On a PC, your phone’s storage can be accessed from Windows Explorer by simply plugging in the USB data/charging cable – no software is required. On your phone’s removable SD card (Card): On your phone’s internal storage (Phone):
![]() In the alchemy room, there is a chalkboard. You need level 2 in Reconstruction, a master builder, and the Alchemy Room puzzle complete. Put the builders on the lift so they start fixing it all at the same time. You need level 2 in Reconstruction on the tech screen and 3 adept builders. Put the leader on the fire and then the 3 adept farmers on as well. You need level 2 in Leadership on the tech screen and at least 3 adept farmers. On the right of the map near the bottom, there are a bunch of leaves on a stone. All of them must be carried back by an adept scientist or better to fix the room. There is a brown pot, a red striped pot, a yello striped pot, a morter, and a stick thing. Get an adept scientist and then put them next to the various pots and items around the village so they carry it back and fix the chamber. Then, next to the beehive are some burnt out torches, put a villager on the torches until they are “Trying an idea….” Then wait patiently until the bees are smoked out. Once they have put the items on the firepit, put a villager on the fire pit to start a fire. Then, you have a leader.īuild a fire by putting a villager on the pile of wood on the beach line and another villager on the pile of dry grass on the very far left of the map near the water fall. The Golden Child will remove the boulder blocking the cave, but only when it's ready to move it you can't force it.Put vilagers next to the robe in the amplitheater until the robe fits one of them. This puzzle requires Level 3 of Construction and Level 3 of Science. You need to place a master builder to find the hiding spot. A clue will not be given by just dragging the villager over the area. Butterflies will appear and follow The Golden Child.ĭiscover buried treasure located south of the food bin, between the bin and the temple. The child will become The Golden Child.Īs long as the previous puzzle is finished, put The Golden Child on the magic garden discovered in Puzzle 9. With Puzzle 5 and 12 completed, and Level 3 of Fertility, put a nursing mother in the lagoon. Then, drag a builder over to the rock near the lagoon, on the west side, to have it chisel an idol out of the rock for the temple. Reach Level 3 of Spirituality and be sure to finish Puzzle 11. Restore the temple at the ruins by dragging a villager to the ruins in the southeast side of the island. Then, drag the Golden Child to the plant north of the berry bush so that the butterflies can pollinate it. They will need to bring water from the lagoon to revive the garden.ĭo Puzzle 14 first and then wait for the butterflies to follow the Golden Child. So long as Puzzle 5 is completed, put a builder villager where they see dead flowers on the east side of the island. Drag a villager to each plant until each is mastered. One is the cactus near the boulder, another is near the lagoon, and two are on the east side of the island. Following the first burial, the villagers will automatically have burial ceremonies for other villagers that die.ĭiscover herbs by researching 4 specific plants on the island. When a villager dies, drag an adult villager to the northeast corner of the island for a proper burial. ![]() With Level 2 Spirituality, find the graveyard. Drag the villager to the rock pile that's blocking the water the water then will flow to the lagoon.Īfter completing Puzzle 5 and having a Level 3 harvester, take the master farmer fishing at the lagoon. The villager needs Level 2 of Construction in order for this to work. ![]() Have a builder remove the blockage from the creek. Just drag the villager over there to have him start cleaning.Ĭonvert a building to a school by dragging a master scientist over to the building. A villager who is trained to build is required.Ĭlean up the debris on the beach so the villagers can hunt for fish. Have a builder remove the water supply's well cover by dragging him to the well.īuild a new hut for villagers to live in. |
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